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. 2000 Mar 1;28(5):1099–1105. doi: 10.1093/nar/28.5.1099

Figure 5.

Figure 5

rRef-1 is a stimulator of Pax-5 DNA-binding activity. DNA-binding by 1 µg of rPax-5 protein, in the absence (lane 3) or presence of 1.5 (lane 7) or 1 µg (lane 8) of rRef-1 or of the reducing agent DTT (5 mM) (lane 2) was analyzed by EMSA. Lane 1 contains 0.5 µg of probe alone; lane 4 contains rPax-5 under reducing conditions without probe; lane 5 contains rRef-1 and rPax-5; lane 6 contains rRef-1 and the DNA probe encompassing the promoter of the sea urchin histon H2A-2.2 (25). The oxidized form of rPax-5 was obtained by prolonged air exposure. The EMSA was developed by silver nitrate staining.