Figure 6.

Effect of Ref-1 on transactivation activity of the Δ71-BSAP promoter. (A) Plasmids were transfected by the calcium phosphate method in HeLa cells at the concentrations indicated in Materials and Methods. Forty-eight hours after transfection, cells were harvested and CAT and Luc activities were measured in nuclear extracts. Bars indicate the means ± SD of at least three independent experiments. (B) Plasmids were transfected by electroporation into NS-1 cells at the concentrations indicated in Materials and Methods. Twenty-four hours after transfection, cells were harvested and CAT and Luc activities were measured in nuclear extracts. Bars indicate the means ± SD of at least three independent experiments.