Time-resolved stomatal conductance in response to 2 uM ABA petiole-fed leaves in wild-type (WT, Col-0), (a-c)
acs octuple and (d-f)
etr1-6;etr2-3 double mutant leaves. Stomatal conductance means (±SEM) of (a-c) n=5 (d-f) n=4 leaves from individual plants per genotype. (b, e) Stomatal conductance (in panels a and d) were normalized to the steady-state stomatal conductance before ABA was applied. (c and f) Changes in absolute stomatal conductance (mean ± SEM) were calculated at the indicated time points based on the data in panels a and d (t1 = steady-state stomatal conductance, t2=20 min and t3=50 min following application of ABA). Statistical analyses were done using unpaired Student’s t tests between the wild-type and the mutant line, P-value is presented under columns. Experimental sets were repeated three times showing similar results.