Fig. 9: ABA-induced stomatal closure is intact in the ethylene receptor hypersensitive triple mutant, etr2-3; ein4-4;ers2-3, while etr1-6 mutant plants show enhanced ABA-induced stomatal closure.

Time-resolved stomatal-conductance in response to 5 uM ABA sprayed leaves in wild-type (WT, Col-0), (a-c) acs octuple (d-f) etr2-3;ein4-4;ers2-3 triple mutant and (g-i) etr1-6. (a, d and g) Stomatal conductance means (±SEM) of n=4 leaves from individual plants per genotype. (b, e and h). Stomatal conductances (in panels a, d and g) were normalized to the steady-state stomatal conductance before ABA was applied). (c, f and i) Changes in absolute stomatal conductance (mean ± SEM) were calculated at the indicated time points based on the data presented in panels a, d and g (t1 = steady-state stomatal conductance, t2=20 min following ABA spray). Statistical analyses were done using unpaired Student’s t tests between the wild-type and the mutant line, P-value is presented above columns. Experimental sets were repeated three times showing similar results.