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. 2023 Jun 6;16(6):dmm049531. doi: 10.1242/dmm.049531

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Microvascular changes within MCA/129 fibrosarcoma tumours post radiation treatment in ASMase^−/− mice. (A) Anti-CD31 antibody staining of endothelial cells lining blood vessels allowed for visualization of MCA/129 mouse fibrosarcoma tumours in WT or S1P-treated mice. Scale bar: 40 μm. (B) Individual vessels were quantified to measure MVD under a 10× objective lens. 0 Gy (n=7), 10 Gy/5F (n=7), 20 Gy/5F (n=5), USMB only (n=5), 10 Gy/5F+USMB (n=5), 20 Gy/5F+USMB (n=6). Error bars represent mean±s.e.m. Statistical analysis by Welch's two-tailed unpaired t-test.