| Strain, strain background (Escherichia coli) |
BL21(DE3) |
Sigma-Aldrich |
CMC0014 |
Electrocompetent cells |
| Cell line (Homo sapiens) |
Human embryonic kidney epithelial |
ATCC |
CRL-11268 |
|
| Cell line (Homo sapiens) |
SK-N-BE(2) neuroblastoma |
ATCC |
CRL-2271 |
|
| Chemical compound, drug |
BRF110 |
Sigma-Aldrich |
CAS 2095489-35-1 |
|
| Chemical compound, drug |
HX600 |
Axon Medchem |
CAS 172705- 89-4 |
|
| Chemical compound, drug |
9-cis-Retinoic acid |
Cayman Chemicals |
CAS 5300-03-8 |
|
| Chemical compound, drug |
Bexarotene |
Cayman Chemicals |
CAS 153559- 49-0 |
|
| Chemical compound, drug |
LG100268 |
Cayman Chemicals |
CAS 153559-76-3 |
|
| Chemical compound, drug |
CD3254 |
Cayman Chemicals |
CAS 196961-43-0 |
|
| Chemical compound, drug |
SR11237 |
Tocris Bioscience |
CAS 146670-40-8 |
|
| Chemical compound, drug |
UVI3003 |
Cayman Chemicals |
CAS 847239-17-2 |
|
| Chemical compound, drug |
LG100754 |
Cayman Chemicals |
CAS 180713-37-5 |
|
| Chemical compound, drug |
IRX4204 |
MedChemExpress |
CAS 220619-73-8 |
|
| Chemical compound, drug |
Rhein |
Sigma-Aldrich |
CAS 478-43- 3 |
|
| Chemical compound, drug |
HX531 |
Cayman Chemicals |
CAS 188844-34-0 |
|
| Chemical compound, drug |
Danthron |
Sigma-Aldrich |
CAS 117-10-2 |
|
| Chemical compound, drug |
PA452 |
Tocris Bioscience |
CAS 457657-34-0 |
|
| Peptide, recombinant protein |
FITC-PGC1α |
LifeTein |
|
Amino acid sequence: EAEEPSLLKKLLLAPANTQ, with an N-terminal FITC label and an amidated C-terminus. |
| Recombinant DNA reagent |
Nurr1-ligand binding domain (LBD) (plasmid) |
de Vera et al., 2016
|
Bacteria expression plasmid |
|
| Recombinant DNA reagent |
RXRα-ligand binding domain (LBD) (plasmid) |
Kojetin et al., 2015
|
Bacteria expression plasmid |
|
| Recombinant DNA reagent |
pET45b(+) |
Novagen |
71327-3 |
|
| Transfected construct (Photinus pyralis) |
3xNBRE-luciferase plasmid |
de Vera et al., 2016
|
Sanger sequenced |
|
| Transfected construct (Photinus pyralis) |
3xDR1-luciferase plasmid |
Hughes et al., 2014
|
Mammalian expression plasmid, Sanger sequenced |
The 3xPPRE-luciferase reporter plasmid in the referenced paper was used in our study. |
| Transfected construct (human) |
Full-length human Nurr1 |
de Vera et al., 2016
|
Mammalian expression plasmid, Sanger sequenced |
|
| Transfected construct (human) |
Full-length human RXRα in pCMV-Sport6 vector |
Zhang et al., 2011c
|
Mammalian expression plasmid, Sanger sequenced |
We obtained this plasmid from Griffin lab at UF Scripps Institute (see referenced paper). |
| Recombinant DNA reagent |
pcDNA3.1 empty vector |
Thermo Fisher Scientific |
V790-20 |
|
| Sequence-based reagent |
RXRα-ΔLBD-F |
This paper |
PCR primer ordered from Sigma |
CAGCAGCGCCTAAGAGGACATG
|
| Sequence-based reagent |
RXRα-ΔLBD-R |
This paper |
PCR primer ordered from Sigma |
CATGTCCTCTTAGGCGCTGCTG
|
| Sequence-based reagent |
ΔNTD-RXRα-F |
This paper |
PCR primer ordered from Sigma |
CCACCCCTCGAGAAACATGG
|
| Sequence-based reagent |
ΔNTD-RXRα-R |
This paper |
PCR primer ordered from Sigma |
CCATGTTTCTCGAGGGGTGG
|
| Gene (human) |
Nurr1 (NR4A2) |
Uniprot |
|
Full length: residues 1–598; LBD: residues 353–598 |
| Gene (human) |
RXRα (NR2B1) |
Uniprot |
|
Full length: residues 1–462; LBD: 223–462 |
| Sequence-based reagent |
Restriction enzymes, ligase for cloning |
NEB |
|
XhoI, HindIII, T4 ligase |
| Commercial assay or kit |
Gibson assembly |
NBE |
E2611L |
|
| Commercial assay or kit |
Britelite plus Reporter Gene Assay System |
Perkin Elmer |
6066769 |
|
| Sequence-based reagent |
RXRα-LBD |
This paper |
gBlock for Gibson assembly |
CCAGCACAGTGGCGGCCGCATGAAGCGGGAAGCCGTGCAGGAGGAGCGGCAGCGTGGCAAGGACCGGAACGAGAATGAGGTGGAGTCGACCAGCAGCGCCAACGAGGACATGCCGGTGGAGAGGATCCTGGAGGCTGAGCTGGCCGTGGAGCCCAAGACCGAGACCTACGTGGAGGCAAACATGGGGCTGAACCCCAGCTCGCCGAACGACCCTGTCACCAACATTTGCCAAGCAGCCGACAAACAGCTTTTCACCCTGGTGGAGTGGGCCAAGCGGATCCCACACTTCTCAGAGCTGCCCCTGGACGACCAGGTCATCCTGCTGCGGGCAGGCTGGAATGAGCTGCTCATCGCCTCCTTCTCCCACCGCTCCATCGCCGTGAAGGACGGGATCCTCCTGGCCACCGGGCTGCACGTCCACCGGAACAGCGCCCACAGCGCAGGGGTGGGCGCCATCTTTGACAGGGTGCTGACGGAGCTTGTGTCCAAGATGCGGGACATGCAGATGGACAAGACGGAGCTGGGCTGCCTGCGCGCCATCGTCCTCTTTAACCCTGACTCCAAGGGGCTCTCGAACCCGGCCGAGGTGGAGGCGCTGAGGGAGAAGGTCTATGCGTCCTTGGAGGCCTACTGCAAGCACAAGTACCCAGAGCAGCCGGGAAGGTTCGCTAAGCTCTTGCTCCGCCTGCCGGCTCTGCGCTCCATCGGGCTCAAATGCCTGGAACATCTCTTCTTCTTCAAGCTCATCGGGGACACACCCATTGACACCTTCCTTATGGAGATGCTGGAGGCGCCGCACCAAATGACTTGATCGAGTCTAGAGGGCCCG
|
| Commercial assay or kit |
LanthaScreen Elite Tb-anti-His antibody |
Thermo Fisher |
#PV5895 |
|
| Software, algorithm |
NITPIC software |
Keller et al., 2012
|
|
Baseline calculation, curve integration |
| Software, algorithm |
SEDPHAT |
Brautigam et al., 2016
|
|
Estimation of binding affinity and thermodynamic parameter measurements |
| Software, algorithm |
GUSSI |
Brautigam, 2015
|
|
Plot ITC figures |
| Other |
NMR chemical shift assignment of Nurr1 LBD |
Michiels et al., 2010; de Vera et al., 2019; Munoz-Tello et al., 2020
|
BMRB16541 |
Published NMR peak assignment from Biological Magnetic Resonance Data Bank |
| Software, algorithm |
NMRFx |
Norris et al., 2016
|
|
NMR data process and analysis |
| Software, algorithm |
NMR LineShapeKin version 4 |
Kovrigin, 2012
|
|
NMR lineshape analysis |
| Software, algorithm |
MATLAB R2022a via NMRbox |
Maciejewski et al., 2017
|
|
NMR lineshape analysis |
| Software, algorithm |
Pearson and Spearman correlation analysis |
GraphPad Prism |
|
Correlation analysis |
| Software, algorithm |
Principal component analysis (PCA); |
GraphPad Prism |
|
Correlation analysis |
| Software, algorithm |
ANOVA multiple comparison test |
GraphPad Prism |
|
Statistical testing |
