Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer extension by dTTP, ddTTP, IIId and Va, catalyzed by TDTP– (Series A, B) and TDTP+ (Series C, D) in the presence of 2 mM Co2+ buffer (Series A, C) or 10 mM Mn2+ buffer (Series B, D). Lane 1, primer + enzyme (control); lanes 2–5, as in lane 1 + 5 µM dTTP, 0.1 µM ddTTP, 0.1 µM Va and 0.1 µM IIId, respectively.
