Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Xenopus laevis) |
Male and female adult frogs | National Xenopus Resource (NXR) Xenopus 1 |
||
| Chemical compound, drug | Human chorionic gonadotropin hormone (hCG) | Sigma-Aldrich | CG10-10VL | |
| Chemical compound, drug | Pregnant mare serum gonadotropin (PMSG) | BioVendor | Catalog # RP1782725000 | |
| Peptide, recombinant protein | cyclinBΔ90 | Wilbur and Heald, 2013 | ||
| Peptide, recombinant protein | UbcH10 C114S | Wilbur and Heald, 2013 | ||
| Antibody | Anti-xCAP-G (rabbit polyclonal) | Susannah Rankin | IF (1:500) WB (1:1000) |
|
| Antibody | Anti-xCAP-G2 (rabbit polyclonal) | Susannah Rankin | IF (1:100) | |
| Antibody | Anti-H1.8 (rabbit polyclonal) | Maresca et al., 2005 | IF (1:1000) WB (1:1000) |
|
| Antibody | Anti-TopoII-a (rabbit polyclonal) | Yoshiaki Azuma | IF (1:1000) WB (1:1000) |
|
| Antibody | Anti-H3, Clone MABI 0301 (mouse monoclonal) | Abcam | Catalog # 39763 |
Whole-embryo IF (1:500) Extract IF (1:1000) |
| Antibody | Anti-E7 beta-tubulin (mouse monoclonal) | Developmental Studies Hybridoma Bank (DSHB) | Link to product | Whole-embryo IF (1:250) |
| Antibody | Alexa Fluor 568 goat-anti-rabbit IgG (polyclonal) | Invitrogen | Catalog # A11011 |
IF (1:1000) |
| Antibody | Alexa Fluor 568 goat-anti-mouse IgG (polyclonal) | Invitrogen | Catalog # A11004 |
IF (1:1000) |
| Antibody | Alexa Fluor 700 goat-anti-rabbit IgG (polyclonal) | Invitrogen | Catalog # A21038 |
WB (1:10,000) |
| Antibody | IRDye-800CW goat anti-mouse IgG (polyclonal) |
LI-COR | Catalog # 926-32210 |
WB (1:10,000) |
| Chemical compound, drug | APT1 Inhibitor (Palmostatin B) | Sigma-Aldrich | Catalog # 178501 |
Reconstitute with DMSO, store at –80°C in aliquots, discard after 3 mo |
| Recombinant DNA reagent | pSNAP/Importin α (WT and NP) | Brownlee and Heald, 2019 |