Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6 J | Jackson Laboratory | Stock #: 000664 | |
| Strain, strain background (M. musculus) | Arg1f/f | Jackson Laboratory | Stock #: 008817 | |
| Strain, strain background (M. musculus) | KF | Wen et al., 2019 | ||
| Strain, strain background (M. musculus) | KFCA | This paper | Pasca di Magliano Lab, University of Michigan | |
| Cell line (M. musculus) | 7940B | Long et al., 2016 | KPC cell line | |
| Antibody | ARG1 (Rabbit, monoclonal) | Cell Signaling, Danvers, MA | Cat #: 93668 S | IF: 1:75 WB: 1:1000 |
| Antibody | CD45 (Mouse, monoclonal) | R&D Systems, Minneapolis, MN | Cat #: MAB14302 | IF: 1:400 |
| Antibody | ECAD (Mouse, monoclonal) | Cell Signaling, Danvers, MA | Cat #: 14472 S | IF: 1:50 |
| Antibody | F4/80 (Rabbit, monoclonal) | Cell Signaling, Danvers, MA | Cat #: 70076 S | IF: 1:250 IHC: 1:250 |
| Antibody | CK19, Troma III (Rat, monoclonal) | Developmental Studies Hybridoma Bank, Iowa City, IA | IF: 1:50 | |
| Antibody | Ki67 (Rabbit, polyclonal) | Abcam, Cambridge, UK | Cat #: ab15580 | IHC: 1:1000 |
| Antibody | CC3 (Rabbit, polyclonal) | Cell Signaling, Danvers, MA | Cat #: 9661 L | IHC: 1:300 |
| Antibody | CD8 (Rabbit, monoclonal) | Cell Signaling, Danvers, MA | Cat #: 98941 S | IHC: 1:300 IF: 1:400 |
| Antibody | APOE (Rabbit, monoclonal) | Abcam | Cat #: ab183597 | IF: 1:500 |
| Antibody | COX1 (Goat, polyclonal) | Santa Cruz | Cat #: Sc-1754 |
IF: 1:200 |
| Antibody | GZMB (Rabbit, monoclonal) | Cell signaling | Cat #: D2H2F | IF: 1:800 |
| Antibody | Vinculin (Rabbit, monoclonal) | Cell signaling | Cat #: 13901 S | WB: 1:1000 |
| Commercial assay, kit | Alexa fluor 488 Tyramide SuperBoost kit | Invitrogen | Cat #: B40922 |
|
| Commercial assay, kit | RNA Scope Multiplex Fluorescent v2 Detection Kit | Advanced Cell Diagnostics | Cat #: 323110 |
|
| Commercial assay, kit | RNAscope Probe-Hs-ARG1 | Advanced Cell Diagnostics | Cat #: 401581 |
|
| Chemical compound, drug | CB-1158 | Calithera Biosciences, Inc, South San Francisco, CA | 100 mg/kg, Oral gavage, twice a day |
|
| Chemical compound, drug | Anti-PD1 | BioXcell | Cat #: BE0033-2, clone J43 |
200 μg/i.p. Every 3 d |
| Software, algorithm | R Studio | Rstudio.com | Version: 4.1.1 -- "Kick Things" | |
| Software, algorithm | ImageJ | Imagej.nih.gov | Version: 2.0.0-rc-69/1.52 p | |
| Software, algorithm | Adobe Illustrator | Adobe.com | 2022 | |
| Software, algorithm | Halo software | Indica Labs | ||
| Software, algorithm | Prism 9 for macOS | Graphpad.com | Version: 9.4.1 (458), July 18, 2022 |