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. 2023 Jun 12;55(6):1066–1075. doi: 10.1038/s41588-023-01421-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. Histogram shows the distribution of estimated cell cycle phase by GPLVM (Online Methods). b. Scatterplots show scaled expression of known cell cycle genes (UBE2C and CDC6) and a gene highly expressed in G0/G1 phase (FN1). The red curves show the posterior mean estimates of expression levels by GPLVM (Online Methods). c. UMAPs of the target cell states coloured by unknown batch or cell cycle phase. UMAP coordinates are identical to Fig. 2a.