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. 2023 Apr 18;14(17):1543–1555. doi: 10.1111/1759-7714.14897

FIGURE 4.

FIGURE 4

Knockdown of circ_PLXND1 suppressed non‐small cell lung cancer (NSCLC) cell development through interacting with miR‐1287‐5p. H1299 and PC9 cells were transfected with si‐NC, si‐circ_PLXND1, si‐circ_PLXND1 + anti‐miR‐NC or si‐circ_PLXND1 + anti‐miR‐1287‐5p. (a) The level of miR‐1287‐5p in H1299 and PC9 cells was examined by qRT‐PCR assay. (b) Tube formation assay was performed to measure the number of formed tubes. (c–e) The proliferation of H1299 and PC9 cells were analyzed by EdU assay (c) and MTT assay (d and e). (f) The protein level of PCNA in H1299 and PC9 cells was measured via Western blot assay. (g–i) The apoptosis (g), migration and invasion (h and i) of H1299 and PC9 cells were evaluated by flow cytometry analysis and transwell assay, respectively. (g and k) The protein levels of Twist1 and E‐cadherin in H1299 and PC9 cells were measured using western blot assay. *p < 0.05.