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. 2023 Apr 18;14(17):1543–1555. doi: 10.1111/1759-7714.14897

FIGURE 5.

FIGURE 5

Circ_PLXND1 could regulate ERBB3 expression through targeting miR‐1287‐5p. (a) The putative binding sites between miR‐1287‐5p and ERBB3 3'UTR were predicted by starBase v2.0. (b and c) Dual‐luciferase reporter assay was conducted to verify the interaction between miR‐1287‐5p and ERBB3. (d–f) The expression of miR‐1287‐5p, ERBB3 mRNA and ERBB3 protein in H1299 and PC9 cells transfected with miR‐NC, miR‐1287‐5p, anti‐miR‐NC or anti‐miR‐1287‐5p was measured by qRT‐PCR assay or western blot assay. (g and h) The mRNA and protein levels of ERBB3 in NSCLC tissues and normal tissues were determined by qRT‐PCR assay and western blot assay, respectively. (i and j) The correlation between the level of ERBB3 mRNA and miR‐1287‐5p or circ_PLXND1 was analyzed by Spearman's correlation coefficient analysis. (k and l) The mRNA and protein levels of ERBB3 in 16HBE, H1299 and PC9 cells were examined by qRT‐PCR assay and western blot assay, respectively. (m and n) The mRNA and protein levels of ERBB3 in H1299 and PC9 cells transfected with si‐NC, si‐circ_PLXND1, si‐circ_PLXND1 + anti‐miR‐NC or si‐circ_PLXND1 + anti‐miR‐1287‐5p were tested by qRT‐PCR assay and western blot assay, respectively. *p < 0.05.