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. 2000 Mar 1;28(5):1228–1236. doi: 10.1093/nar/28.5.1228

Figure 1.

Figure 1

GST–HMG1 forms a complex with RAG1 and RAG2 on 12RSS DNA. This EMSA shows protein–DNA complexes resolved under native conditions in the absence of competitor DNA. Two probes are used: the 12RSS probe (lanes 1–7) and an equivalent probe in which the nonamer element has been mutated (lanes 8–14). The RAG proteins MR1 and MR2 are present in lanes marked with a plus sign at the top of the figure. GST–HMG1 is present in varying concentrations except in lanes 1 and 8. Complexes consisting of a single GST–HMG1 molecule per probe DNA or two molecules per DNA are indicated as HMG1 (1) and HMG1 (2) respectively. The complex formed by the RAG proteins without HMG1 is indicated as MR1+2. The complex containing all three proteins is marked as MR1+2+HMG1. The figure is a composite from two parallel gels.