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UV crosslinking of the RAG proteins to 12RSS and 23RSS probes under specific binding conditions. (A) 12RSS probes were assembled into complexes in the presence of MR1, MR2 and GST–HMG1 and crosslinked in solution. Complexes were then analyzed by SDS–PAGE. No correction for interference in complex assembly was applied to this image. (B) Selected 23RSS probes were used as above. Probe 18 is located in the 23RSS coding DNA, 21 in the heptamer and 24 in the nonamer.
