Figure 2.

Localization of the transactivation domain of GKLF using its cognate binding site. CHO cells were co-transfected with 5 µg/10 cm dish each of the TDAx2-E_1bTATALUC reporter and the respective effector constructs containing various regions or mutations of GKLF (effectors 1–7) or the vector PMT3 (V) alone. (A) Means of fold-induction in reporter activity over that of the vector (n = 4). (B) Schematic of the various effector constructs. The three E*s in effector 6 indicate the mutated glutamate residues and the D*s in effector 7 indicate the three mutated aspartate residues.