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In vivo competition of GKLF’s transactivation by E1A-expressing constructs. CHO cells were transfected with 2 µg/10 cm dish of pG5TKLUC, 0.5 µg/10 cm dish of GAL4-GKLF(1–109) and increasing amounts of a competitor RSV construct containing either wild-type or mutated E1A in fold-excess relative to that of GAL4-GKLF(1–109). The relative luciferase activity of cells that received no competitor DNA was chosen as 100%. n = 3 in all experiments. CR indicates conserved region.
