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. 2023 Mar 28;9(4):720. doi: 10.18063/ijb.720

Figure 7.

Figure 7.

TP53 and BRAF mutations detected in MDA-MB-231 cells isolated by BA-LIFT technology. (A) Agarose gel of PCR reaction products from the amplification of exon 9 of the TP53 in a MDA-MB-231 cell (lane 1), 25 MDA-MB-231 cells (lane 2), 1000 MDA-MB-231 control (lane 3), 1000 PBMCs control (lane 4), and negative control (lane 5) compared to a DNA ladder (lane L). (B) Electropherogram showing the partial sequence of exon 9 of TP53 in a MDA-MB-231 cell (B.1), 25 MDA-MB-231 cells (B.2), 1000 MDA-MB-231 cells control (B.3), and 1000 PBMCs control (B.4). The presence of thymine and absence of guanine at position 839 of exon 9 of TP53 gene (homozygous) can be observed in cases B.1, B.2, and B.3. The mutation is not observed in B.4. (C) Agarose gel of PCR reaction products from the amplification of exon 11 of BRAF gene in a MDA-MB-231 cell (lane 1), 25 MDA-MB-231 cells (lane 2), 1000 MDA-MB-231 control (lane 3), 1000 PBMCs control (lane 4), and negative control (lane 5) compared to a DNA ladder (lane L). (D) Electropherogram showing the partial sequence of exon 11 of BRAF gene in a MDA-MB-231 cell (D.1), 25 MDA-MB-231 cells (D.2), 1000 MDA-MB-231 cells control (B.3), and 1000 PBMCs control (B.4). The presence of guanine and thymine at position 1391 of exon 11 of BRAF gene (heterozygous) can be observed in cases B.1, B.2 and B.3. The mutation is not observed in B.4.