Fig. 6.
Knockdown of specific PDE4D isoforms increases neurite length and protects against Aβ1-42-induced neurite length reduction. HT22 cells were transfected with CRISPR-Cas9 vectors to genetically knock down specific PDE4D isoforms. A Average neurite lengths upon PDE4D isoform knockdown 48 h after transfection (**P < 0.01, ***P < 0.001, ****P < 0.0001, Dunnett’s post hoc t tests; n = 9–12/condition). B Average neurite lengths upon PDE4D isoform knockdown 48 h after transfection with 1 µM Aβ1-42 exposure during the last 24 h (****P < 0.0001, Dunnett’s post hoc t tests compared to control with Aβ1-42 exposure; n = 9/condition). Comparisons against control transfection without Aβ1-42 exposure are shown by hashes (##P < 0.01, ####P < 0.0001, Dunnett’s post hoc t tests). Data are presented as mean + SEM