Figure 7.

Restriction digest analysis of injected pCAT-Eco72I. Eco72I digestion of pCAT-Eco72I injected into Xenopus oocytes was evaluated in manually separated nuclei. Labeled, circular pCAT-Eco72I was added to non-injected (NI) samples (whole cell extract or nuclei) as a control for the Eco72I activity. Nuclei were subjected to increasing concentrations of Eco72I to ensure that the enzyme was not limiting. The fragments digested with both Eco72I and PstI were separated at 1917 bp, while the single digested fragment appeared at 4782 bp. The data were quantitated by electronic autoradiography (InstantImager; Packard Instrument Co., Meriden, CT).