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HeLa cells also contain the 70 kDa protein that binds to the human rDNA promoter in the presence of human SL1-fraction. Human TFIA-, Pol I-, UBF- and SL1-fractions were prepared from HeLa whole-cell extract as described. A UV-cross link experiment was performed essentially as described in Figure 2 with the photoreactive probe corresponding to the human rDNA promoter region from –56 to –1.
