Figure 6.
Transcription activity of partially purified p70 on the rDNA transcription in vitro. Transcription reactions were done in the presence or absence of 3.6 µg of Pol I-fraction (lanes 4, 6, 8, 9 and 10), 1.0 µg of SL1/UBF-fraction (lanes 5, 7, 8, 9 and 10) and 2.0 (lane 9) or 4.0 µg (lanes 3, 6, 7 and 10) of p70-fraction. In vitro transcribed RNA of 320 nt was produced as in the control reaction with the nuclear extract (lane 1).