Table 2.
The optimized conditions, specific activity, and radiochemical purity of [89Zr]Zr-Trastuzumab
| Optimized conditions for radiolabeling | time | 30 min | 1 h | 1.5 h | 1 h |
| temperature | Room temp | 37°C | 37°C | 37°C | |
| pH | 7.4 | 7.0 | 7.1 | 7.0 | |
| Chelator/mAb molar ratio | - | - | 5:1 | 10:1 | |
| Radiochemical purity investigation | Method/mobile phase | ITLC/0.1 M EDTA | RTLC, 50mM DTPA | RTLC, 50mM DTPA | RTLC, 20 mM citric acid |
| percentage | - | 98.7% | > 98% | ||
| Specific Activity | 50 GBq/g | 136.9 GBq/g | 22.2-74 GBq/g | 74 GBq/g | |
| reference | (20) | (19) | (15) | This study | |