Skip to main content
. 2000 Mar 1;28(5):1045–1052. doi: 10.1093/nar/28.5.1045

Figure 4.

Figure 4

NORA protein expression. (A) Western blot analysis. pHis-NORAfull was expressed in E.coli and the total protein extract was electrophoresed on a 12% SDS–polyacrylamide gel, transferred to a nitrocellulose membrane and detected by the Penta-His antibody. Relative migration of size standards is shown on the right side of the panel. (B) In vitro translation. Rabbit reticulocyte lysates were used to translate His-NORAfull cRNA in [35S]methionine labelled proteins. A radioautograph of a 12% polyacrylamide gel is shown with molecular weights of markers in kDa shown on the right.