98 °C	30 s
98 °C	10 s	Test 16–22 cycles. In our hands, libraries for Input and strong RBPs, such as PTBP1 and SRSF1, often need less than 16 cycles without over-amplification
65 °C	30 s
72 °C	30 s
72 °C	3 min
10 °C	hold