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. 2023 Jun 12;6(9):e202201732. doi: 10.26508/lsa.202201732

Figure 6. MiR-93-5p regulating PDCD4 expression repressed neuronal apoptosis through activating PI3K/Akt pathway in acute ocular hypertension (AOH) retina.

Figure 6.

(A, B, C) Western blot was performed to detect the expression levels of PI3K, Akt, and p-Akt in AOH retina. Retinal PI3K, Akt, and p-Akt expression was significantly reduced in AOH injury (**P < 0.01, #P < 0.05, ##P < 0.01, one-way ANOVA, n = 3). Overexpression of miR-93-5p increased the expression of these proteins. These molecules showed no significant change in miR-93-5p NC+AOH group compared with AOH group. (D, E, F) Western blot was carried out to examine the expression levels of PI3K, Akt, and p-Akt in AOH retina. Intravitreal siPDCD4 injection of AOH increased PI3K, Akt, and p-Akt expression compared with the AOH group (**P < 0.01, #P < 0.05, ##P < 0.01, one-way ANOVA, n = 3). However, injection of siRNA NC did not have any obvious effect on these molecules in AOH retinal injury. All animals were euthanized 3 d after model establishment. Data were presented as the mean ± s.d. Compared with the control: **P < 0.01. Compared with the AOH, miR-93-5p NC+AOH and siRNA NC+AOH group: #P < 0.05, ##P < 0.01.