Abstinence from IntA to oxycodone was associated with reduced phosphorylated DAT at Threonine-53 (pDAT) on abstinence day (AD)15. (A) Representative blots for Naïve, AD2 and AD15 groups. (B) Total DAT (tDAT), (C) pDAT, (D) pDAT/tDAT ratio and (E) Mu opioid receptor (MOR) in the NAc. Naïve, n = 6 (3 females and 3 males); AD2, n = 7 (3 females and 4 males); AD15, n = 8 (4 females and 4 males). Data shown are mean ± SEM. *p < 0.05 compared to Naïve.