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. 2023 Jan 31;119(6):1336–1351. doi: 10.1093/cvr/cvad024

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© The Author(s) 2023. Published by Oxford University Press on behalf of the European Society of Cardiology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Experimental protocols of myocardial I/R injury and various ischaemic conditioning methods in rats. (A) Budapest study centre—hind-limb ischaemia and reperfusion by clamping femoral artery and vein. (B) Szeged study centre—hind-limb ischaemia and reperfusion by tightening and loosening of a tourniquet. (C–E) Experimental protocol with various durations of myocardial ischaemia in the Amsterdam study centre—hind-limb ischaemia and reperfusion by using pressure cuff. Initial group sizes (n) as the number of animals are shown under the corresponding groups. LAD: left anterior descending coronary artery; RIC: remote ischaemic conditioning; CON: control; IPC: ischaemic preconditioning; UNIRIC: unilateral RIC; BIRIC: bilateral RIC; IS: infarct size; MVO: microvascular obstruction; BP: blood pressure; HR: heart rate.