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. 2022 Dec 20;119(6):1361–1376. doi: 10.1093/cvr/cvac191

Figure 1.

Figure 1

CARMEN-201 controls SMC specification via its second exon. (A and B) Representative images and quantification of ACTN2-positive TNNI-positive CMs and SMMHC-positive SMCs in differentiating fetal and adult CPC cultures. Scale bar: 50 µm. (C) Annotated CARMEN isoforms. The CARMEN-201second exon is highlighted. (D) Absolute quantification of CARMEN-201 (C-201), CARMEN-205 (C-205) and CARMEN-217 (C-217) in differentiating fetal and adult CPCs. (E) Nuclear and cytoplasmic levels of CARMEN-201, CARMEN-205, CARMEN-217, ACTB, and NEAT. (F) Expression of CARMEN-201, CARMEN-205, CARMEN-217, SMC markers (MYH11; CNN1; TAGLN), and CM markers (MYH6; MYH7) in adult WT and Δ201Ex2 CPC clones lacking CARMEN-201 Exon 2. (G and H) Representative images and quantification of SMMHC-positive CNN1-positive TAGLN-positive SMCs in cultures of differentiating adult WT or Δ201Ex2 CPC clones. Scale bar: 50 µm. Data represent mean values ± SEM; *P < 0.05 as compared with fetal CPCs in expansion; §P < 0.05 compared with the indicated conditions (n = 3–6). ANOVA with post hoc Tukey. See also see Supplementary material online, Figure S1–S3.