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. 2022 Dec 20;119(6):1361–1376. doi: 10.1093/cvr/cvac191

Figure 3.

Figure 3

Transcriptomic analysis and identification of upstream regulators of CM and SMC specification in fetal CPCs with or without C-201 Exon 2 overexpression. (A) Principal Component Analysis (PCA) visualizing transcriptomic data in a two-dimensional space. (B) Expression heatmap of regulators and markers of the CM and the SMC fate. The heatmaps show scaled TPM values. (C and D) TRIAGE transformation of input RNA Seq data predicts regulatory genes controlling cell differentiation based on TRIAGE rank order (left) compared with ranking observed using simple gene expression (right). Control differentiation (None) vs. differentiation following C-201 Exon 2 overexpression (C-201 Ex2) 1 (d1) or 7 days (d7) after transduction. Regulators implicated in cardiovascular differentiation are highlighted in red. (E) Human heart single-cell data analysis reveals genes positively and negatively correlated with CARMEN expression during development. (F) UMAP plots showing epicardium-, pericyte-, and SMC-specific expression of CARMEN in the human heart 6.5 weeks post-conception. See also Supplementary material online, Figure S4 and S5.