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. 2022 Dec 20;119(6):1361–1376. doi: 10.1093/cvr/cvac191

Figure 6.

Figure 6

Validation of candidate cardiogenic factor downregulation. (A–D) Expression of epicardial genes (WT1, TCF21, TBX18) in differentiating fetal CPCs following IRX1, IRX5, ISL1, and SFRP1 silencing. (E) Epicardial (WT1, TCF21, TBX18) SMC (MYH11, TAGLN, CNN1) and CM marker (MYH6) expression in differentiating fetal CPCs overexpressing C-201 Ex2, and in fetal CPCs treated with siRNAs directed against the indicated factors in combination. (F) Representative images and quantification of ACTN2-positive CMs and SMMHC-positive CNN1-positive SMCs in cultures of differentiating fetal CPCs transfected as in (E). Scale bars: 50 µm. Expression of CARMEN-201 (C-201), CARMEN-205 (C-205) and CARMEN-217 (C-217) in differentiating fetal CPCs treated as in (E). Data represent mean values ± SEM; *P < 0.05 as compared with fetal CPCs in expansion; §P < 0.05 as compared with fetal CPCs in differentiation (n = 6–12). ANOVA with post hoc Tukey. See also see Supplementary material online, Figure S8.