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. 2000 Mar 1;28(5):1211–1220. doi: 10.1093/nar/28.5.1211

Figure 2.

Figure 2

Southern analysis of L.major genomic DNA. (A) Partial restriction map of the lmpab1 locus. Regions encoding the RNA binding domains and the C-terminal domain are boxed and highlighted differentially. Positions of the initiator and stop codons and extent of probes B and C are shown. Internal restriction sites are indicated: N, NotI; P, PstI; X, XhoI. (B) Southern hybridisation of probes B and C to L.major genomic DNA digested with XhoI (lanes 1 and 6); PstI (lanes 2 and 7); NotI (lanes 3 and 8); EcoRI (lanes 4 and 9); ClaI (lanes 5 and 10). Blots were hybridised and washed at low stringency, as described.