Figure 4.

RNA binding analysis. (A) Binding of cytoplasmic parasite proteins to end-labelled poly(A). Reactions were performed at low (0.15 M) or high salt (1 M NaCl); unlabelled poly(A) competitor was added as indicated to reactions containing low salt. Complexes were UV cross-linked and separated by 10% SDS–PAGE followed by autoradiography. N, no lysate added. (B) Immunoprecipitation of LmPAB1:poly(A) complexes bound at low (0.15 M) or high (1 M) salt, with anti-LmPAB1 or with pre-immune serum (Pre), followed by 10% SDS–PAGE separation and autoradiography (4 days at –70°C).