| Gene (human) |
GLP1R |
Integrated DNA Technologies |
|
|
|
Strain, strain background
(Escherichia coli) |
NEB 10-beta Competent E.
coli
|
NEB |
C3019 |
|
| Commercial assay or kit |
NEBuilder HiFi |
NEB |
E2621 |
|
| Recombinant DNA reagent |
Pfu-Ultra II Fusion |
Agilent |
600387 |
|
| Cell line (Mus
musculus) |
GLUTag enterocrine cell line |
Daniel J Drucker (Univ. of
Toronto) |
|
|
| Cell line (Homo
sapiens) |
Human Embryonic Kidney (HEK293T) |
ATCC |
CRL-3216 |
|
| Antibody |
Anti-FLAG-Alexa-647 |
M1 (Sigma-Aldrich) In-house conjugated
to Alexa-647 (Miriam Stoeber, University of Geneva) |
F3040 |
IF(1:1000) |
| Chemical compound |
Fmoc- and side chain-protected L-amino
acids |
Bachem AG |
|
|
| Chemical compound |
Piperidine |
Chemie Brunschwig AG |
110-89-4 |
99% |
| Chemical compound |
O-(7-Azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium
hexafluorophosphate (HATU) |
Bachem AG |
148893-10-1 |
|
| Chemical compound |
(7-Azabenzotriazol-1-yloxy)tripyrrolidinophosphonium
hexafluorophosphate (PyAOP) |
Advanced ChemTech CreoSalus |
156311-83-0 |
|
| Chemical compound |
N,N-Diisopropylethylamine
(DIPEA) |
Sigma-Aldrich Chemie GmbH |
7087-68-5 |
|
| Chemical compound |
Trifluoroacetic acid (TFA) |
Sigma-Aldrich Chemie GmbH |
76-05-1 |
For HPLC, ≥99.0% |
| Chemical compound |
Triisopropylsilane (TIPS) |
Sigma-Aldrich Chemie GmbH |
6485-79-6 |
98% |
| Chemical compound |
3,6-Dioxa-1,8-octane-dithiol
(DODT) |
Sigma-Aldrich Chemie GmbH |
14970-87-7 |
95% |
| Chemical compound |
α-Methyl-5-methoxy-2-nitro-4-(2-propyn-1-yloxy)benzyl alcohol |
Sigma-Aldrich Chemie GmbH |
1255792-05-2 |
|
| Chemical compound |
N,N'-Disuccinimidyl
carbonate (DSC) |
Sigma-Aldrich Chemie GmbH |
74124-79-1 |
|
| Other |
NovaPEG Rink Amide resins (0.41 mmol/g
and 0.20 mmol/g loading) |
Sigma-Aldrich Chemie GmbH |
|
|
| Other |
AldraAmine trapping packets (volume
1000–4000 mL) |
Sigma-Aldrich Chemie GmbH |
|
|
