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. 2023 Jun 13;5(3):zcad029. doi: 10.1093/narcan/zcad029

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© The Author(s) 2023. Published by Oxford University Press on behalf of NAR Cancer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — WEE1 and PKMYT1 co-inhibition exacerbates replication stress and triggers replication catastrophe. (A) Dose response matrix for QIBC analysis of replication stress upon 4 h treatment with adavosertib in combination with RP-6306 in U2OS cells. AU = arbitrary unit; RS = replication stress; RC =replication catastrophe. (B) Analysis of relative cell population percentage from panel (A). (C) Line plots for CDK activity (FOXM1pT600), chromatin-bound RPA (CB-RPA) and γH2AX in indicated treatments. (D) QIBC plots related to panel (C) for CB-RPA and γH2AX. (E) QIBC plots related to panel (C) for FOXM1pT600 and DAPI; red indicates γH2AX-positive cells; AU = arbitrary unit. (F) Western blot analysis of soluble and chromatin fractions following indicated treatments of U2OS cells; CDK1i = RO-3306, n = 2.