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. 2023 May 22;6(6):857–867. doi: 10.1021/acsptsci.2c00180

Figure 8.

Figure 8

Effect of peptide on SARS-CoV-2 replication in Calu-3 cells. (A) Schematic representation of replication kinetics of SARS-CoV-2 (Delta variant; lineage: B.1.617.2 and Omicron variant; lineage: BA.1.529) measured in Calu-3 cells treated with the peptide. Representative immunofluorescence images of SARS-CoV-2-Delta variant infected (positive control), or uninfected (negative control) Calu-3 cells treated without or with different concentrations of peptide (1.56 to 0.48 μM). (B) Cells were probed with antibodies against SARS-CoV-2 S protein (green). Scale bar; 100 mm. Percentage inhibition SARS-CoV-2 infection Delta (C) and Omicron (D) were quantified from immunofluorescence results. SARS-CoV-2 viral load Delta (E) and Omicron (F) was quantified by RT-qPCR in Calu-3 cell culture supernatant treated without or with different concentrations of peptide and IC50 was determined. (G) Cytotoxicity of peptide was measured in Calu-3 cells for 48 h. Data represents mean SEM from three replicas.