Figure 4.

Another variant of a mutation analysis as it is shown in Figure 3. α-S-dGTP and α-S-dATP are used in this primer extension reaction. The mutant allele is observed at 1560 Da and the wild-type allele at 1576 Da. The top is the spectrum of an individual who is wild type, the bottom an individual carrying the mutation on one chromosome. There is a 1 Da difference between the wild-type alleles of the two analyses. This is due to rounding of the raw data. It represents the largest difference detected in this study.