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Antibody dilutions for western blot and immunofluorescent staining of human pancreatic ductal epithelial cells
| Antibody | Dilution |
|---|---|
| Rabbit H3K27me3 | 1:2500 |
| Mouse total H3 | 1:1000 |
| Rabbit H3K27ac | 1:1000 |
| Mouse β-actin | 1: 10,000 |
| Rabbit EZH2 | 1:1000 |
| IRDye® 680CW goat anti-rabbit | 1:10,000 |
| IRDye® 800CW goat anti-mouse | 1:10,000 |
| Rabbit anti-CK19 | 1:200 |
| Guinea pig anti-insulin | 1:250 |
| Alexa fluor 488 donkey anti-rabbit | 1:1000 |
| IRDye® 680CW donkey anti-guinea pig | 1:1000 |
