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(a) Decarboxylation of malonyllysine to acetyllysine. (b) No decarboxylation of malonyllysine is seen at room temperature. However, heat can cause chemical decarboxylation of malonyllysine-containing proteins to form acetyllysine, a cross-reactive epitope. Note that higher malonyllysine signal is also observed after heating, likely due to increased reaction of proteins with malonyl-NAC.
