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. Author manuscript; available in PMC: 2023 Jun 14.
Published in final edited form as: Chembiochem. 2021 Nov 5;23(1):e202100491. doi: 10.1002/cbic.202100491

Table 1.

Michaelis-Menten kinetic parameters for malonyllysine-ACC and malonyltetrazole-ACC substrate hydrolysis by SIRT5.

Ac-LGK(Mal)-ACC Ac-LGK(Tet)-ACC
kcat (s−1) (3.83 ± 0.15) × 10 −3 (1.79 ± 0.08) × 10 −4
KM (μM) 80.26 ± 9.18 15.73 ± 2.87
Vmax (μM S−1) (3.83 ± 0.15) × 10 −3 (5.38 ± 0.23) × 10 −4
kcat/KM(M−1 S−1) 47.76 ± 16.11 11.40 ± 2.66