Figure 2.

Anatomical distribution of MNPs in neonatal and adult PP

(A) FACS quantification of CD4⁺ T cells and phagocytic cells in PP. Ratio of mean T cell:phagocyte is indicated.

(B) Sample preparation and signal distribution model of CD11c using radial segmentation of the PP to obtain Δ.

(C) Δ calculated in individual PP (n = 25–36 follicles, Kruskall-Wallis test).

(D, E, and G) Representative spectral confocal imaging projection of PP illustrating anatomical distribution of (D) TLR3⁺IRF8⁺CD11c⁺CX3CR1⁻ cDC1 (TLR3, orange; IRF8, magenta; GFP, green; CD11c, red; CD4, blue [only TLR3⁺ IRF8⁺ cells represent cDC1]), (E) SIRPα⁺CD11c⁺CX3CR1⁻lysozyme⁻ cDC2 (SIRPα, magenta; EpCAM, gray; GFP, green; CD11c, red; lysozyme, yellow; CD4, blue [arrowheads point toward cDC2 in the SED]) and (G) RORγt⁺CD11c⁺CX3CR1⁻CD4⁻ APC (RORγt, magenta; GFP, green; CD11c, red; lysozyme, yellow; CD4, blue [boxed areas of individual RORγt⁺APC shown below]). Scale bars, 20 μm.

(F) Ratio between SIRPα⁺CX3CR1⁺Lysozyme⁺CD11c⁺ MC and SIRPα⁺CX3CR1⁻lysozyme⁻CD11c⁺ cDC2 in SED shown in (E). (n = 5–6, median, Mann-Whitney U test.) Each dot represents one animal (A) or one follicle (C and G). See also Figure S2.