Figure 5.

Cells were treated with 0.1 μM of rhodamine-labeled stapled peptide targeting PD-L1 in the presence or absence of MG132. (A) After 4 h, the PD-L1 level was detected by western blotting and quantitative analysis of the protein level in C33A and HeLa. Values are presented as mean ± SEM. One-way ANOVA followed by Tukey’s post-hoc test compared to control (n = 3). (B) After 4 h, the distribution of peptides in cells was observed by confocal laser microscopy. Red, peptide; blue, nucleus. Scale bar, 10 μM. ***P < 0.001.