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. 2023 Jun 13;14:3500. doi: 10.1038/s41467-023-39147-4

Fig. 3. Protease preference of BA.5 S protein is comparable to that of BA.1 and BA.2.

Fig. 3

A Inhibition of S protein-mediated cell entry by protease inhibitors. Target cells were preincubated (1 h, 37 °C) in the presence of no inhibitor, MDL28170 (20 µM), camostat (20 µM) or a combination of MDL28170 and camostat (20 µM each) before pseudoviruses were added. Inhibition of cell entry was analyzed. The average (mean) data ± SEM from three biological replicates (each with four technical replicates) are presented, with entry standardized against no inhibitor-treated cells (set as 0% inhibition). B Concentration-dependent inhibition of S protein-mediated cell entry by MDL28170 and camostat. Target cells were preincubated (1 h, 37 °C) in the presence of no inhibitor or different concentrations of MDL28170 or camostat before pseudoviruses were added. Inhibition of cell entry was analyzed. The average (mean) data ± SEM from three biological replicates (each with four technical replicates) are presented, with entry standardized against no inhibitor-treated cells (set as 0% inhibition). Statistical analyses: two-way analysis of variance with Dunnett’s post-hoc test was used to determine statistical significance compared to no inhibitor-treated cells (p > 0.05, not significant [ns]; p ≤ 0.05, *; p ≤ 0.01, **; p ≤ 0.001, ***), see also Extended Data Table 3.