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. 2023 Jun 13;3(6):1026–1040. doi: 10.1158/2767-9764.CRC-22-0415

FIGURE 2.

FIGURE 2

Immune subtypes based on TIL profiling using FCM; NAT (n = 157), LUAD [85; Cold (n = 19), Myeloid (n = 36), CD8 (n = 30)], LUSQ [50; Cold (n = 14), Myeloid (n = 19), CD8 (n = 17)]. A, Heat map of cell densities and %CD45 of immune cells in NSCLC tumors and NATs. Each column represents a patient; data were normalized by Z-scores to indicate relative compositions of immune cells. Cell density is presented as the log of each cell type. %CD45 is presented as each immune cell type/CD45. Heat maps of cell density and %CD45 of TILs in LUAD (B) and LUSQ (C) tissues. D, Cell densities and %CD45 of immune cell types in LUAD and LUSQ immune subtypes. Top, Cell density indicates immune cells. Bottom, %CD45 indicates immune cells. AD.Cold, AD.Myeloid, and AD.CD8 are LUAD subtypes Cold, Myeloid, and CD8, respectively. SQ.Cold, SQ.Myeloid, and SQ.CD8 are LUSQ subtypes Cold, Myeloid, and CD8, respectively. E, IHC staining for CD8 in a representative case from immune subtypes. For CD8, FOXP3, CD20, and Ki67, the positivity of each marker was given as the number of total positive cell number within each tissue core. For CD33, the ratio of CD33-positive staining area per tissue area was calculated. F, Immune cell counts by IHC staining in lung cancer tissues. G, CD8+ T cell–attractant chemokine gene expression in immune subtypes.