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. 2023 Apr 21;150(8):dev200995. doi: 10.1242/dev.200995

Fig. 3.

Fig. 3.

TEC phenotypes in K5.Foxn1;Foxn1Z/Z mice. All data were collected from 1-month-old mice. (A) FACS profile of gated CD45−EpCam+ epithelial cells from 1-month-old thymi stained for MHCII and UEA-1. (B) Frequencies of TEC subsets based on UEA-1 and MHCII staining of EpCam+ TECs from +/Z, K5.Foxn1Tg+;+/Z, Z/Z and K5.Foxn1Tg+;Z/Z thymus. *P<0.05, **P<0.01 (n=5). (C) Ratio of mTECs:cTECs in +/Z, K5.Foxn1Tg+;+/Z, Z/Z and K5.Foxn1Tg+;Z/Z thymus. **P<0.01 (n=5). (D) Cryosections stained for AIRE (green). The number and intensity of AIRE+ cells were decreased in Z/Z mutants, and rescued by transgene expression. (E) Fluorescence intensity measurements show that AIRE levels were rescued by the K5.Foxn1 transgene in Z/Z thymus. ***P<0.005 (n=7). (F) Percentage of AIRE+ cells in gated CD45−EpCam+UEA-1+ thymic epithelial cells. (G) Gating strategy for AIRE+ cells in CD45−EpCam+UEA-1+ thymic epithelial cells. **P<0.01, ***P<0.005 (n=5). Statistical analyses were carried out using one-way ANOVA with multiple comparison testing. Data are mean±s.e.m. NS, not significant. Scale bar: 50 µm. All paired images are shown at same magnification.