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. 2023 Apr 21;150(8):dev200995. doi: 10.1242/dev.200995

Fig. 4.

Fig. 4.

Thymocyte phenotypes were partially rescued by K5.Foxn1 expression in Foxn1Z/Z mutants. Mice are 6 months old, except in G (n=8). (A) Profile of CD4 and CD8 expression in +/Z, Tg+;+/Z, Z/Z and Tg+;Z/Z thymocytes. (B) The percentage of CD4- and CD8-expressing cells in +/Z, Tg+;+/Z, Z/Z and Tg+;Z/Z thymocytes. *P<0.05, **P<0.01. (C) Profile of CD25 and CD44 expression in gated CD4−CD8− thymocytes. (D) Graph of the percentage of CD25- and CD44-expressing CD4−CD8− cells. *P<0.05, **P<0.01, ***P<0.005 (n=8). Red box highlights increased population in Z/Z thymus. (E) Gated lin−CD25−CD44+ thymocytes stained for CD24 and CD117 (Kit) expression. (F) Graph of the percentage of CD117+CD24hi and CD117+CD24lo cells (DN1ab cells) in +/Z, Tg+;+/Z, Z/Z and Tg+;Z/Z mice. **P<0.01 (n=8). (G) RT-PCR analysis of total DLL4 expression in sorted TEC subsets from +/Z, Tg+;+/Z, Z/Z and Tg+;Z/Z thymi. Mice are 1 month old. *P<0.05, **P<0.01, ***P<0.005 (n=6). Statistical analyses were carried out using one-way ANOVA with multiple comparison testing. Data are mean±s.e.m. NS, not significant.