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. 2023 Apr 21;150(8):dev200995. doi: 10.1242/dev.200995

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© 2023. Published by The Company of Biologists Ltd

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Fig. 5. — Regulatory T cells increase in K5.Foxn1 thymus but not in K5.Foxn1;Foxn1^Z/Z mutants. (A) FOXP3 in gated CD45⁺ thymocytes. (B) The percentage of FOXP3⁺ cells in gated CD45⁺ thymocytes. **P<0.01 (n=8). (C) Immunostaining for FOXP3 on paraffin sections of thymus. (D) Profile of CD25 and FOXP3 expression in gated CD4⁺ thymocytes. (E) The percentage of FOXP3⁺ cell in gated CD4⁺ thymocytes. *P<0.05, **P<0.01 (n=8). Statistical analyses were carried out using one-way ANOVA with multiple comparison testing. Data are mean±s.e.m. NS, not significant. Scale bar: 100 µm. All paired images are shown at same magnification.