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. 2023 Apr 19;150(8):dev201531. doi: 10.1242/dev.201531

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — mCerulean as a versatile blue fluorophore for zebrafish transgenesis. (A) The stable transgenic line for HOPX^4q12N3.1_minprom:mCerulean,crybb1:mKate2 shows mCerulean expression in both the vacuolar and sheath cells of the zebrafish notochord at 2 days post-fertilization and eye lens-specific mKate2 expression (asterisk) when crossed with the drl:mCherry transgenic, marking lateral plate mesoderm-derived lineages (cardiovascular system, blood at 2 dpf). (B,C) Transient injection of zebrafish ubiquitin (ubb) promoter-driven mCerulean with 3′ polyadenylation trailer (polyA) from SV40 (B) or ubb (C). (D-F) mCerulean constructs for different cellular localization. Transient expression of cytoplasmic mCerulean (D), nuclear nls-mCerulean (E) and membrane-bound mCerulean-CAAX (F). Scale bars: 500 μm (A-C); 50 μm (D-F).