Table 1.
Summary of Photophysical Properties of the NorCy7 Series of Compounds
λmax,abs/λmax,em (nm) |
εmax (M−1 cm−1) |
pKa | ΦFd(%) | |
---|---|---|---|---|
Sulfo-NorCy7a | 755/775 | 73 500 | 5.2 | 8.9 |
H-NorCy7b | 750/770 | 63 900 | 5.8 | 9.1 |
OMe-NorCy7b | 775/790 | 56 800 | 5.5 | 1.6 |
Pip-NorCy7c | 760/780 | 17 200 | 4.4 | 8.1 |
N-Me-Pip-NorCy7a | 760/775 | 45 000 | 4.5 | 10 |
N-Me-NorCy7a | 730/750 | 57 600 | 4.6 | 12 |
Acetate buffer pH 4.5.
MeOH/acetate buffer pH 4.5 (2:1).
MeOH/acetate buffer pH 3.75 (2:1).
MeOH + 0.1% formic acid for all compounds.