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MCU Inhibitor RU360 prevents uptake of Ca2+. (a) and (b) 2 mg of mitochondria were incubated with or without mitochondrial calcium uniporter (MCU) inhibitor 10 μM Ru360 and subjected to the CRC/swelling assay. Untreated (Unt.) mitochondria are able to take up Ca2+ until the MPTP engages, while Ru360 treated mitochondria are unable to intake Ca2+ and never undergo MPTP opening
