Figure 3.

Myc-independent initiation and elongation of tsp-1 mRNA. (A) Nuclear run-off assay performed with Rat-1A (left columns) and Rat-1A/LMycSN (right columns) cells. Probes used were the full-length human tsp-1 cDNA (‘tsp1-full’), exons 1 (‘tsp1-Ex1’) and 22 (‘tsp1-Ex22’) of the rat tsp-1 gene, rat gapdh cDNA (‘gapdh’) and the empty cloning vector pGEM (‘vector’). (B) RNase protection analyses performed on total (left) and nuclear (right) RNAs from Rat-1A and Rat-1A/LMycSN cells. The probes represented exons 1 and 22 of the rat tsp-1 gene and the rat gapdh gene (loading control). Electrophoretic mobility of the molecular mass markers (MspI fragments of the plasmid pBR322) is shown on the right.